JS1057: Biotin-PEG2-NHS 596820-83-6
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发布时间:2021.12.22 新闻来源:品科研-生物试剂,生化试剂,高端试剂,试剂超市-河北品科研生物科技有限公司官网 浏览次数:836 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. JS1057, Biotin-PEG2-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 40 mg of antibodies.
Preparation:
Use of a 10 mM Biotin-PEG2-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-PEG2-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.
制备:
建议使用10 mM生物素化溶液。为了有效地对样品进行生物素化,生物素化溶液应高于15倍的含胺蛋白的摩尔量。 来计算一下10 mM生物素化溶液量(见下面的示例)。
Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM JS1057 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]
A = 20 [uL of 10 mM JS1057 solution]
计算: A μL 10 mM生物素化溶液
用于生物素化2 mg IgG(150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [μL 10 mM 生物素化溶液] x 10-6 [L/μL] x 10 [mmol/L] x 10-3 [mol/mmol]
A = 20 [μL 10 mM 生物素化溶液]
Procedure:
1. Bring Biotin-PEG2-NHS (Product No. JS1057) to room temperature.
2. Dissolve 2 mg of Biotin-PEG2-NHS (Product No. JS1057) in 400uL of PBS and prepare a 10 mM solution of Biotin-PEG2-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-PEG2-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.
使用说明:
1. 将每种产品置于室温。
2. 将2 mg生物素-PEG2-NHS [JS1057]溶于 400μL PBS中,制备10 mM生物素化溶液。
3. 将样品(1-10 mg/mL)溶解在适当的缓冲液中,如PBS。不要使用含有胺的缓冲液(如Tris)。
4. 向样品溶液中加入A μL 10 mM生物素化溶液
5. 在室温下将混合溶液培养30分钟。 6. 用脱盐柱或透析法除去未反应和水解的试剂。 订购方式 1、直接联系公司客服4000685696订购 2、支持各大试剂交易平台线上订购
3、各大高校、科研院所内部采购平台搜索货号“JS1057-1SET”订购
4、公司汇款信息 名称:河北品科研生物科技有限公司 纳税人识别号:91130681MA0EJ81G2Q 地址:河北省保定市涿州市开发区朝阳路和谷产业园A1-5-59号 电话:17710261641 开户行:中国工商银行涿州支行 账号: 0409020009300376204
JSENB品牌简介 JSENB品牌的創立者,香港吉斯恩貝國際貿易有限公司。
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